The labs work on the entire chain of drug discovery for acute and chronic nervous system diseases, from the study of the causes of the disease to therapy including through the application of the latest technologies of regenerative medicine (cell therapies and new materials). The specific skills include modeling and clinical study of diseases of interest.
The morphology laboratory studies the proteins, peptides, trophic factors and receptors distribution in distinct regions of the central and peripheral nervous system, using multiple fluorescence localization techniques.
Relevant Instruments: Leica CM1950
Molecular biology lab analyzes the genes of interest starting from cells or microdissected tissues, and it permits the development of tools for gene expression manipulation.
Relevant Instruments: real time PCR Stratagene Mx3005P, BioRad RT-PCR CFX-96, Ultracentrifuge Beckman Optima MAX E, laser capture microdissection to miniCellcut of microscope Nikon Eclipse TE2000-S
Cell culture lab works on cell lines and primary cultures of nerve and glial cells. It includes a section dedicated to the study of neural stem cells and other types of stem cells.
Relevant Instruments: laminar flow hoods, incubators, life imaging microscope of Olympus
Proteomics lab permits to measure simultaneously protein and peptides panels in the same sample of fluid and organic extracts, using multiparameter technologies. Proteonomics lab can partecipate to clinical studies.
Relevant Instruments: Luminex 200 and Luminex MAGPIX
Microscopy and image analysis lab is equipped with the latest imaging technologies, dynamic imaging and computerized image analysis (4D space-time).
Relevant Instruments: Nikon A1 confocal laser scanning, also configured for time-lapse experiments on multiple points, form FRAP, FRET and calcium imaging; fluorescence miscoscopy equipped with high resolution CCD camera; 2D, 3D and 4D image analysis softwares (Image ProPlus, Olympus Cell^P, Nikon NIS, Bitplane Imaris).
Animal behavioral lab studies spontaneous cognitive and sensorimotor behavior
Relevant Instruments: video-recording apparatus, video-tracking and automatic behavior analysis software
The laboratory has an internal network, with stand-alone server and back-up certificate data to external media and in extra-cop.